Human ACE2-Functionalized Gold "Virus-Trap" Nanostructures for Accurate Capture of SARS-CoV-2 and Single-Virus SERS Detection

作者：Yang, Yong^*; Peng, Yusi; Lin, Chenglong; Long, Li; Hu, Jingying; He, Jun^*; Zeng, Hui; Huang, Zhengren^*; Li, Zhi-Yuan; Tanemura, Masaki; Shi, Jianlin; Lombardi, John R.^*; Luo, Xiaoying^*

来源：Nano-Micro Letters, 2021, 13(1): 109.

DOI：10.1007/s40820-021-00620-8

• SERS
• SARS-CoV-2
• Human ACE2
• "Virus-trap" nanostructure
• Single-virus detection

The current COVID-19 pandemic urges the extremely sensitive and prompt detection of SARS-CoV-2 virus. Here, we present a Human Angiotensin-converting-enzyme 2 (ACE2)-functionalized gold "virus traps" nanostructure as an extremely sensitive SERS biosensor, to selectively capture and rapidly detect S-protein expressed coronavirus, such as the current SARS-CoV-2 in the contaminated water, down to the single-virus level. Such a SERS sensor features extraordinary 10(6)-fold virus enrichment originating from high-affinity of ACE2 with S protein as well as "virus-traps" composed of oblique gold nanoneedles, and 10(9)-fold enhancement of Raman signals originating from multi-component SERS effects. Furthermore, the identification standard of virus signals is established by machine-learning and identification techniques, resulting in an especially low detection limit of 80 copies mL(-1) for the simulated contaminated water by SARS-CoV-2 virus with complex circumstance as short as 5 min, which is of great significance for achieving real-time monitoring and early warning of coronavirus. Moreover, here-developed method can be used to establish the identification standard for future unknown coronavirus, and immediately enable extremely sensitive and rapid detection of novel virus.

• 单位
  上海交通大学; 中国科学院; 中国科学院研究生院; y