Europium (III) chelate nanoparticle-based lateral flow immunoassay strips for rapid and quantitative detection of cystatin C in serum

作者：Bian, Lun; Xiong, Yufeng; Zhao, Hui; Guo, Huiling; Li, Zhaoyue; Ye, Ke; Zhang, Zhigao; Liu, Tiancai; Wu, Yingsong^*; Lin, Guanfeng^*

来源：Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences , 2022, 1194: 123133.

DOI：10.1016/j.jchromb.2022.123133

• Renal failure
• Glomerular filtration rate
• Cystatin C
• Europium-chelate nanoparticles
• Lateral flow immunoassay

The concentration of Cys C in the patient's serum can reflect the level of glomerular filtration rate and indicate the occurrence of renal failure. The establishment of a simple and rapid analytical method to quantitatively monitor the concentration of Cys C in serum could help timely detection of renal failure. In this study, we have developed an Eu (III) chelate nanoparticles based lateral flow immunoassay to fulfill real-time monitoring of Cys C concentration in serum within 15 min. This method was performed as a sandwich immunoassay with a wide detection range (0.05-10 mu g/mL) and a low limit of detection (24.54 ng/mL). The intra and inter-assay coefficients of variation were 8.31-8.61% and 8.92-9.95%, respectively. Furthermore, the application of this method was evaluated by comparing the determined results with those obtained by chemiluminescence immunoassay, exhibiting a satisfactory correlation (R-2 = 0.9830). The developed LFIA method with satisfactory analytical performance has great potential for real-time monitoring of renal failure and self-detection for the high-risk population.& nbsp;& nbsp;

• 单位
  中山大学; 南方医科大学