Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2

作者：Zhang, Ke; Liu, Chi; Hu, Changbin; Lin, Ping; Qi, Qi; Jia, Huizhen; Tang, Jiebing^*; Yu, Xiaoguang^*

来源：Heliyon, 2023, 9(6): e16870.

DOI：10.1016/j.heliyon.2023.e16870

• Prostate cancer
• LncRNA AC245100
• 4
• Proliferation
• PAR2
• PI3K
• AKT pathway

Background: Prostate cancer (PCa) is among the most generally diagnosed cancers in males. A long non-coding RNA (lncRNA) called AC245100.4 has been discovered and linked to PCa carcino-genesis. However, its specific and potential mechanism is uncertain in PCa. In this research, we investigated the role of AC245100.4 in cell proliferation and the underlying mechanism in PCa cells.Methods: qRT-PCR assays were utilized to detect AC245100.4 expression and confirm its down-stream target. The pathways related to AC245100.4 were identified by RAP-MS. PCa cell pro-liferation was experimented by Cell Counting Kit-8 and Colony formation assays. Western blot was performed to detect PAR2, AKT, p-AKT, Cyclin D1 and PCNA expression.Results: AC245100.4/PAR2 overexpression promotes PCa cell proliferation and the opposite re-sults are obtained after AC245100.4/PAR2 knockdown. Mechanistically, we found that PAR2 is confirmed as the AC245100.4 downstream target and AC245100.4 promotes PCa cell prolifera-tion by regulating PAR2. AC245100.4 promotes PCa cell proliferation via PI3K/AKT pathway. Rescue assays validated that PAR2 knockdown reversed the impact of AC245100.4 over -expression on increasing p-AKT protein levels. Conclusion: This research revealed that AC245100.4 enhances cell proliferation in PCa cells through modulating the PAR2/PI3K/AKT axis, which may offer novel tumor markers and po-tential therapeutic targets for PCa.

• 单位
  哈尔滨医科大学