Enhance fluorescence study of grating structure based on three kinds of optical disks

摘要

Based on the grating structure of discarded optical disks, a fluorescent substrate with simple operation, excellent performance, and low cost were synthesized and applied to the detection of toxic molecule crystal violet (CV). The effect of the width and depth of the grating on the fluorescence signal was explored by using cleverly the natural structural differences of the three kinds of optical disks and the method of magnetron sputtering. To obtain the best substrate, two kinds of fluorescent molecules were used to screen and verify the microstructure of different morphologies. Besides, the three-dimensional finite-difference time-domain (FDTD) method is used to analyze the intensity of the local electromagnetic field and the size of the "hot spots"area of the three kinds of optimal substrates. The experimental results show that the substrates have excellent stability (reduced by 7.26% after one week) and reproducibility (RSD ≤4.85%), and the detection of CV molecule also shows good performance (LOD = 10−10 M). Therefore, the substrate has great potential value in biological and chemical sensing, and it can also provide a new design idea for the study of surface enhanced fluorescence (SEF) substrate.

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