Large-scale analysis of 2,152 Ig-seq datasets reveals key features of B cell biology and the antibody repertoire

作者：Yang, Xiujia; Wang, Minhui; Wu, Jiaqi; Shi, Dianchun; Zhang, Yanfang; Zeng, Huikun; Zhu, Yan; Lan, Chunhong; Deng, Yang; Guo, Shixin; Xu, Lijun; Ma, Cuiyu; Zhang, Yanxia; Ou, Jinxia; Liu, Chu-Jun; Chen, Yuan; Wang, Qilong; Xie, Wenxi; Guan, Junjie; Ding, Jieyu; Wang, Zhi; Chang, Changqing; Yang, Wei; Zhang, Huijie; Chen, Jun; Qin, Lijie; Zhou, Hongwei; Bei, Jin-Xin; Wei, Lai; Cao, Guangwen; Yu, Xueqing; Zhang, Zhenhai

来源：Cell Reports, 2021, 35(6): 109110.

DOI：10.1016/j.celrep.2021.109110

• HUMAN-IMMUNOGLOBULIN HEAVY
• PLASMA-CELLS
• IMMUNE REPERTOIRE
• GENE REPERTOIRE
• BLOOD
• DIVERSITY
• INFECTION
• IDENTIFICATION
• VACCINATION
• MUTATIONS

Antibody repertoire sequencing enables researchers to acquire millions of B cell receptors and investigate these molecules at the single-nucleotide level. This power and resolution in studying humoral responses have led to its wide applications. However, most of these studies were conducted with a limited number of samples. Given the extraordinary diversity, assessment of these key features with a large sample set is demanded. Thus, we collect and systematically analyze 2,152 high-quality heavy-chain antibody repertoires. Our study reveals that 52 core variable genes universally contribute to more than 99% of each individual's repertoire; a distal interspersed preferences characterize V gene recombination; the number of public clones between two repertoires follows a linear model, and the positive selection dominates at RGYW motif in somatic hypermutations. Thus, this population-level analysis resolves some critical features of the antibody repertoire and may have significant value to the large cadre of scientists.

• 单位
  南方医科大学; 广东省人民医院; 华南农业大学; 中山大学; 青岛大学; 浙江大学