Efficient A-to-C base editing with high specificity

Authors：Chen, Liang^*; Li, Dali

Source：Nature Biotechnology, 2023.

DOI：10.1038/s41587-023-01866-w

Generating A-to-C transversions in specific targets via base editing technology has been challenging. By fusing an evolved alkyladenine DNA glycosylase with an engineered adenine deaminase TadA-8e variant and nickase Cas9, we have developed A-to-C base editors that generate precise and efficient A-to-C transversions in cells and in mouse embryos, expanding the possible applications of base editing.