Inhibition mechanism of cyclo (L-Phe-L-Pro) on early stage Staphylococcus aureus biofilm and its application on food contact surface

作者：Li, Hong; Li, Changzhu; Ye, Yong; Cui, Haiying^*; Lin, Lin^*

来源：Food Bioscience, 2022, 49: 101968.

DOI：10.1016/j.fbio.2022.101968

• Cyclo (L-phe-L-pro)
• Staphylococcus aureus
• Quorum sensing
• Polysaccharide intercellular adhesion
• Anti-biofilm mechanism

Staphylococcus aureus (S. aureus) biofilms are of considerable interest in food safety due to the increase risk of the food contamination. This study aimed to explore the anti-biofilm mechanisms of cyclo (L-Phe-L-Pro) (cFP) against S. aureus with respect to its changes on extracellular polymeric substances (EPS) and gene expression. Results showed that cFP decreased the viability of S. aureus biofilm, and inhibited the synthesis of EPS (extra -cellular proteins, extracellular polysaccharides, and extracellular DNA). Real-time qPCR revealed that cFP could affect the expression of polysaccharide intercellular adhesion (PIA) synthesis-related genes (icaR, icaA, icaB icaC and icaD) and quorum sensing-related genes (agrA, agrB, agrC, agrD, geh, hla and spa). In addition, molecular docking speculated that cFP could bind to AgrC, and the binding region was most likely between loop1 and loop2. The histidine kinase domain of AgrC may also played a role in the binding process. Finally, the application of cFP on food contact surface showed that cFP could inhibit the biofilm of S. aureus. These findings not only prove the practicable antibacterial activity of cFP, but provide new approach to decrease the risk of S. aureus contamination on food contact surface.

• 单位
  江苏大学