Water-soluble fluorescent probes for differentiating cancer cells and normal cells by tracking lysosomal viscosity

作者：Liu, Minghui; Weng, Jintao; Huang, Shumei; Yin, Wenjin; Zhang, Huatang^*; Jiang, Yin^*; Yang, Liu^*; Sun, Hongyan^*

来源：Chemical Communications, 2023, 59(24): 3570-3573.

DOI：10.1039/d3cc00359k

Lysosomal viscosity is a significant parameter of lysosomes and closely related to various diseases. Herein, two fluorescent probes, Lyso-vis-A and Lyso-vis-B, were developed, which demonstrate diverse advantages, including great water solubility, lysosome targeting ability and viscosity sensitivity. In particular, Lyso-vis-A exclusively showed fluorescence response toward viscosity but was not influenced by pH changes, rendering it a selective lysosomal viscosity probe. Furthermore, Lyso-vis-A was successfully applied to monitor lysosomal viscosity variations in living cells and differentiate cancer cells and normal cells.

• 单位
  y; 广东工业大学