Synthesis, Antibacterial Activity, and Molecular Docking Study of Bispyrazole-Based Derivatives as Potential Antibacterial Agents

Authors：Ali Salman, Ghazwan; S. Zinad, Dhafer; Mahal, Ahmed^*; Rizki Fadhil Pratama, Mohammad; Duan, Meiato; Alkhouri, Anas; Alamiery, Ahmed

Source：ChemistrySelect, 2022, 7(4): e202103901.

DOI：10.1002/slct.202103901

• Antibacterial Activity
• Drug Discovery
• Medicinal Chemistry
• Molecular Docking
• Pyrazole

Development of novel antibacterial agents is one of most important aims in the field of medicinal chemistry and drug discovery. A new series of bispyrazole derivatives were synthesized with moderate to excellent yields ranging from 68 to 83 %. The structures of the newly synthesized compounds were confirmed using NMR, GC, IR techniques. The prepared derivatives were screened against Gram-positive bacteria (S. aureus and B. subtilis) and Gram-negative bacteria (E. coli and P. aeruginosa). All of the new compounds exhibited good to moderate antibacterial activity against Gram-positive and Gram-negative bacteria compared to trimethoprim. The bispyrazole bearing (triflouromethyl)benzene 8 d showed significant antibacterial inhibition against all bacteria tested and was found to be more active than trimethoprim in term of inhibition zone. Minimum inhibitory concentration (MIC) showed that the bispyrazoles bearing (methylsulfonyl)benzene 8 c and 4-chlorobenzen 8 e showed potent inhibition activity against Staphylococcus aureus and Bacillus subtilis while The bispyrazole bearing (triflouromethyl)benzene 8 d displayed strong inhibitory activity against S. aureus, B. subtilis, E. coli and P. aeruginosa compared to trimethoprim. Molecular docking was investigated for the active compounds and showed promising lead-like characters. These compounds could be employed as prospective lead compounds for the synthesis of novel antibacterial agents with highly potency.

• Institution
  Southern Medical University; Chinese Academy of Science