Expression of napA, nirK, cnorB and nosZ in Chelatococcus daeguensis TAD1 at high temperature under oxic conditions

作者：Yang Yunlong^*; Huang Shaobin

来源：Research Journal of Biotechnology, 2014, 9(9): 38-45.

• Denitrification gene
• oxic expression
• high temperature
• Chelatococcus daeguensis TAD1
• aerobic denitrification

Pure cultures of Chelatococcus daeguensis TAD1 were incubated at high temperature (50 degrees C) using nitrate as a substrate and an electron acceptor for denitrification. The periplasmic nitrate reductase gene (napA), nitrite reductase gene (nirK), nitric oxide reductase gene (cnorB) and nitrous oxide reductase gene (nosZ) expression were measured using a quantitative reverse transcription-PCR at various initial dissolved oxygen (DO) concentrations. All the four genes expression reached a maximum at 12h. The maximum expression level of napA, nirK and nosZ was at 50rpm, the corresponding DO concentrations of which were 2.2 mg/l, but the maximum expression level of cnorB was at 150rpm, the corresponding DO concentrations of which were 2.6 mg/l. These results demonstrated that low dissolved oxygen conditions were more beneficial to expression of the denitrification genes in C. daeguensis TAD1 at high temperature.

• 单位
  华南理工大学