Electron transfer and electrocatalytics of cytochrome c and horseradish peroxidase on DNA modified electrode

作者：Song Y; Wan L; Wang Y; Zhao S; Hou H; Wang L^*

来源：Bioelectrochemistry, 2012, 85: 29-35.

DOI：10.1016/j.bioelechem.2011.11.007

• Cytochrome c
• DNA
• Electron transfer
• Horseradish peroxidase
• Hydrogen peroxide

A bio-interphase composed of DNA, cytochrome c (Cyt c) and horseradish peroxidase (HRP) was developed by layer-by-layer assembling Cyt c, DNA and Cyt c-HRP on biocompatible 11-mercaptoundecanoic acid - 6-mercapto-1-hexanol modified gold electrode. The new bio-interphase was used as a model system to mimic the electron transfer and electrocatalytic performance of two proteins in living organisms. Results showed that the electron transfer rate at bi-protein bio-interphase was faster than those at the single protein bio-interphase, indicating a synergistic interaction between the two proteins occurred in the electron transfer. Moreover, the mixed proteins modified electrode exhibited good electrocatalytic response to reduction of hydrogen peroxide (H 2O 2) and oxygen (O 2), suggesting that it could be used as a sensor for H 2O 2 and O 2 detection. The properties of the bio-interphase, together with the bioelectrocatalytic activity, could make it useful in the development of bioelectronic devices, and investigation of electrochemistry of other heme proteins at functional interphase. It would also provide a new strategy for further study on the electron transfer of other multi-proteins in a bio-interphase and the development of biosensors.