Aggregation-Induced Emission Luminogen-Based Dual-Mode Enzyme-Linked Immunosorbent Assay for Ultrasensitive Detection of Cancer Biomarkers in a Broad Concentration Range

作者：Shen, Jianlei; Situ, Bo; Du, Xianchao; Wang, Zhiming; Hu, Rong; Li, Baixue; Qin, Anjun^*; Tang, Ben Zhong^*

来源：ACS Sensors, 2022, 7(3): 766-774.

DOI：10.1021/acssensors.1c02237

• aggregation-induced emission
• enzyme-linked immunosorbent assay (ELISA)
• dual-mode ELISA
• linear dynamic range
• biomarkers

The enzyme-linked immunosorbent assay (ELISA) is one of the mostcommonly used methods for measuring antibodies and antigens in biological samples.However, developing new ELISAs with high detection sensitivity and broad detectiondynamic ranges without resorting to complicated signal processing and equipmentsetups remains a challenge. In this work, we report a strategy to simultaneouslyimprove the detection sensitivity and broaden the dynamic range by replacing thechromogenic reagents used in traditional ELISAs with an aggregation-inducedemission luminogen (AIEgen). The developed AIE-ELISA could generatecomplementary absorbance andfluorescence signals with a linear detection range of1.6-25,000 pg/mL. The application of this dual-mode AIE-ELISA in the detection ofthe prostate-specific antigen (PSA) realized a limit of detection of 1.3 pg/mL (3.78x10-14M) and dynamic range improvement of approximately 2 orders of magnitudecompared to a single-mode ELISA, which enabled it to discriminate a minor PSAdifference in a patient's serum. The simpler experimental operation, faster enzymeresponse speed, and better photostability of AIEgen than the traditional chromogenic reagents used in ELISAs showed that ourdeveloped AIE-ELISA holds great potential in thefields of immunoassay, immunohistochemistry, and immunocytochemistry.

• 单位
  上海交通大学; 南方医科大学