Rigid and Photostable Shortwave Infrared Dye Absorbing/Emitting beyond 1200 nm for High-Contrast Multiplexed Imaging

作者：Wei, Ruwei; Dong, Yan; Wang, Xueli; Li, Jin; Lei, Zuhai; Hu, Zhubin; Chen, Jinquan; Sun, Haitao; Chen, Hao; Luo, Xiao; Qian, Xuhong; Yang, Youjun^*

来源：Journal of the American Chemical Society, 2023, 145(22): 12013-12022.

DOI：10.1021/jacs.3c00594

• HEPTAMETHINE CYANINE DYES
• BILE-DUCT INJURY
• INDOCYANINE-GREEN
• LAPAROSCOPIC CHOLECYSTECTOMY
• ORGANIC FLUOROPHORES
• SYMMETRY-BREAKING
• BRIGHT
• RHODAMINE
• WAVELENGTH
• DESIGN

The shortwave infrared (SWIR) spectral region beyond1200 nm offersoptimal tissue penetration depth and has broad potential in diagnosis,therapy, and surgery. Here, we devised a novel class of fluorochromicscaffold, i.e., a tetra-benzannulated xanthenoid (EC7). EC7 absorbs/emits maximally at 1204/1290 nm in CH2Cl2 and exhibits an unparalleled molar absorptivityof 3.91 x 10(5) cm(-1) M-1 and high transparency to light at 400-900 nm. It also exhibitedhigh resistance toward both photobleaching and symmetry breaking dueto its unique structural rigidity. It is feasible for in vivo bioimagingand particularly suitable to couple with the shorter-wavelength analoguesfor high-contrast multiplexing. High-contrast dual-channel intraoperativeimaging of the hepatobiliary system and three-channel in vivo imagingof the intestine, the stomach, and the vasculature were showcased. EC7 is a benchmark fluorochrome for facile biomedical exploitationof the SWIR region beyond 1200 nm.

• 单位
  复旦大学; 华东理工大学; 中国科学院