Changes in the TCR repertoire of T-cell subsets during culture of cytokine-induced killer cells

作者：Liang, Guozheng; Feng, Gang; Chen, Hui; Li, Ling; Hu, Jicong; Zhou, Chuandong; Li, Jiawen; Shen, Han; Wu, Fenglin; Tao, Changli; Liu, Yan^*; Zhang, Wenfeng^*; Shao, Hongwei^*

来源：the and of llh 2019 for、with、to\ of the 、 or the\the、or、of d &、&、希基准, 2022, 596(20): 2696-2705.

DOI：10.1002/1873-3468.14501

• CIK
• clonal diversity
• immunoscope
• T-cell subset
• TCR repertoire

Cytokine-induced killer (CIK) cells are a group of highly heterogeneous T cells expressing diverse T-cell antigen receptors (TCRs), but possessing wide major histocompatibility complex (MHC)-unrestricted antitumour activity, which have been used in the treatment of many tumours. However, the clonal diversity of different T-cell subsets and whether there is a certain clonal preference during CIK culture remains to be clarified. We found that the proportion of oligoclones seen in some subfamilies of freshly isolated T cells decreased in varying degrees after culturing. This indicates that the diversity of T-cell clones had been restored to a certain extent. The diversity differed among different T-cell subsets, as some TCR subfamily oligoclones mainly appeared in CD3(+)CD56(+) cells, which also indicates the heterogeneity of the CIK cell composition.

• 单位
  y; 广东药学院; 华南农业大学