The role of hybridization in improving the immune response and thermal tolerance of abalone

作者：Liang Shuang; Luo Xuan; You Weiwei; Luo Lianzhong; Ke Caihuan^*

来源：Fish and Shellfish Immunology, 2014, 39(1): 69-77.

DOI：10.1016/j.fsi.2014.04.014

• Hybridization
• Abalone
• Mortality
• Immune response
• HSP70

Recently, frequent death of cultured abalone drew our attention to the stress tolerance of abalone. Hybridization is an effective way of genetic improvement in aquaculture, which can introduce improved traits to the hybrids. In this study, we challenged the hybrids between Haliotis discus hannai and Haliotis gigantea, and their parents with bacteria (vibrio harveyi, vibrio alginolyticus and vibrio parahemolyticus), then held them at 20 degrees C and 28 degrees C, survival rates of the parental populations and hybrid populations were recorded. Then we tested the immune responses and thermal-induced responses of the four populations at different temperatures. Total hemocyte count (THC), respiratory burst, superoxide dismutase activity (SOD), acid phosphatase activity (ACP), alkaline phosphatase activity (AKP), myeloperoxidase activity (MPO), and HSP70 expression were determined on day 1 and day 7 of the temperature exposure. Results showed higher survival rates of the hybrids than their parents against bacteria challenge. For immune parameters, THCs were evaluated at 28 degrees C, while increased THC was also observed in H. discus hannai female x H. gigantea male (DG) and H. discus hannai female x H. discus hannai male (DD) at 12 degrees C (day 7); at 28 degrees C, respiratory burst was activated (day 1 and 7), while SOD activity first rose then fell over 7-days exposure; AKP activity was elevated at 12 degrees C and 28 degrees C (day 1), most notably in DG, and an increased level of ACP was observed in DG at 28 degrees C (day 7); MPO activity was suppressed at 12 degrees C and 28 degrees C on day 1, but recovered on day 7. For HSP70, increased HSP70 levels were observed in all populations at 28 degrees C (day 1), and DD got the lowest HSP70 level after 7-days exposure at 28 degrees C. Overall, the results suggest that temperature changes could significantly affect the physiological status of abalone, and hybrids may be more resistant to disease and thermal stresses than their parents.

• 单位
  厦门大学