The magic of unraveling genome architecture and function

作者：Cosma, Maria Pia^*; Neguembor, Maria Victoria^*

来源：Cell Reports, 2023, 42(4): 112361.

DOI：10.1016/j.celrep.2023.112361

• CHROMATIN ORGANIZATION
• CHROMOSOME DYNAMICS
• IMAGING REVEALS
• CTCF
• DOMAINS
• COHESIN
• GENE
• DNA
• 3D
• COMPARTMENTS

Over the last decades, technological breakthroughs in super-resolution microscopy have allowed us to reach molecular resolution and design experiments of unprecedented complexity. Investigating how chromatin is folded in 3D, from the nucleosome level up to the entire genome, is becoming possible by "magic"(imaging genomic), i.e., the combination of imaging and genomic approaches. This offers endless opportunities to delve into the relationship between genome structure and function. Here, we review recently achieved objec-tives and the conceptual and technical challenges the field of genome architecture is currently undertaking. We discuss what we have learned so far and where we are heading. We elucidate how the different super-resolution microscopy approaches and, more specifically, live-cell imaging have contributed to the under-standing of genome folding. Moreover, we discuss how future technical developments could address remaining open questions.

• 单位
  广东省人民医院; 南方医科大学