Influence of Dietary Cholesterol on Growth Performance, Fat Accumulation, and Expression of OlApoA-I with Response to Immune Stimuli in Medaka (Oryzias latipes)

作者：Al Hafiz, Md A.; Liang, X. T.; Yao, Q.; Shen, H.; Nibona, E.; Wang, B.; Wang, Z. Q.; Zhong, X. P.; Zhou, Q. Ch; Zhao, H. B.^*

来源：Inland Water Biology, 2022, 15(5): 674-683.

DOI：10.1134/S199508292205008X

• Medaka
• ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I
• mRNA expression
• Cholesterol
• Growth
• Immune stimuli

Apolipoprotein A-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I (ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I) is critical for normal cholesterol metabolism and atheroprotection in mammals. It also involves the immune functions in mammals including humans and fishes. Medaka (Oryzias latipes), a small fish suitable as a model for the study of ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I. In this study, the expression profile of medaka ApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I (OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I) was done in adult tissues and embryos. Moreover, the response of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I to dietary cholesterol and immune stimuli was examined. OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA was expressed in all adult tissues assessed with notably high levels in the intestine and liver. Expression was also detected from the blastula stage to the hatching of embryos using RT-PCR. A cholesterol-rich diet did not affect the growth of young fish but resulted in significant fat deposition in the abdomen. This diet decreased intestinal OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA levels at first but increased towards the end of the study. Conversely, OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA in muscle and fat initially increased, then decreased; OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA was down-regulated in the liver. Further, immune stimulation with lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (PolyI:C) induced up or down-regulation of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I mRNA in immune organs such as liver, intestine, kidney, and spleen in adult fish. The results suggested that OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I is ubiquitously expressed in adult tissues and is a zygotic gene in medaka embryos. The response of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I expression to dietary cholesterol and immune stimuli hints a possible role of OlApoA-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I in cholesterol metabolism and immune response.