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A novel detection of single-stranded DNA binding protein based on ss-DNA modified chip using surface plasmon resonance microscopy

Jian Quan Lua; Ming Bo Xu a; b; Xing Wang Zhou a; Jin Guang Xu a; Qing Tao a aHubei Key Laboratory of Bioanalytical Technique; Hubei Normal University; Huangshi; China bDepartment of Chemistry; Central China Normal University; Wuhan; China
中国知网
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摘要

An ss-DNA gold chip was prepared based on self-assembly of the thiol-derivatized oligonucleotide,and used for thedetermination of single-stranded binding protein(SSB)by surface plasmon resonance microscopy(SPR).The experiment resultsshowed that SSB binds ss-DNA with high specificity,and relative signal of SPR response is proportional to the concentration of SSBin the range of 0.1-100 ng/mL with a detection limit(S/N=3)of 0.07 ng/mL.

关键词

Detection Single-stranded DNA binding protein ss-DNA Surface plasmon resonance microscopy