Nanostructured materials as a host matrix to develop robust peroxidases-based nanobiocatalytic systems

作者：Bilal, Muhammad^*; Ashraf, S. Salman; Romanholo Ferreira, Luiz Fernando; Cui, Jiandong; Lou, Wen-Yong; Franco, Marcelo; Iqbal, Hafiz M. N.^*

来源：International Journal of Biological Macromolecules, 2020, 162: 1906-1923.

DOI：10.1016/j.ijbiomac.2020.08.122

• Nano-bio-catalysis
• Nanostructured materials
• Peroxidases
• Enzyme immobilization
• Carbon nanomaterials
• Metal nanomaterial
• Metal-organic framework
• Catalytic features

Nanostructured materials constitute an interesting and novel class of support matrices for the immobilization of peroxidase enzymes. Owing to the high surface area, robust mechanical stability, outstanding optical, thermal, and electrical properties, nanomaterials have been rightly perceived as immobilization matrices for enzyme immobilization with applications in diverse areas such as nano-biocatalysis, biosensing, drug delivery, antimicrobial activities, solar cells, and environmental protection. Many nano-scale materials have been employed as support matrices for the immobilization of different classes of enzymes. Nanobiocatalysts, enzymes immobilized on nano-size materials, are more stable, catalytically robust, and could be reused and recycled in multiple reaction cycles. In this review, we illustrate the unique structural/functional features and potentialities of nanomaterials-immobilized peroxidase enzymes in different biotechnological applications. After a comprehensive introduction to the immobilized enzymes and nanocarriers, the first section reviewed carbonaceous nanomaterials (carbon nanotube, graphene, and its derivatives) as a host matrix to constitute robust peroxidases-based nanobiocatalytic systems. The second half covers metallic nanomaterials (metals, and metal oxides) and some other novel materials as host carriers for peroxidases immobilization. The next section vetted the potential biotechnological applications of the resulted nanomaterials-immobilized robust peroxidases-based nanobiocatalytic systems. Concluding remarks, trends, and future recommendations for nanomaterial immobilized enzymes are also given.