Exploration of intermolecular interaction of calf thymus DNA with sulfosulfuron using multi-spectroscopic and molecular docking techniques

作者：Shi Jie-Hua^*; Lou Yan-Yue; Zhou Kai-Li; Pan Dong-Qi; Shi JH^*

来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy , 2018, 204: 209-216.

DOI：10.1016/j.saa.2018.06.054

• Sulfosulfuron
• Calf thymus DNA
• Groove binding
• Spectroscopy
• Molecular modeling

As a sulfonylurea herbicide, sulfosulfuron is extensively applied in controlling broad-leaves and weeds in agriculture. It may cause a potential risk for human and herbivores health due to its widely application and residue in crops and fruits. The study of the binding characteristics of calf thymus DNA (ct-DNA) with sulfosulfuron was performed through a series of spectroscopic techniques and computer simulation. The experimental results showed sulfosulfuron interacted with ct-DNA through the groove binding. The negative values of thermodynamic parameter (Delta H degrees, Delta S degrees and Delta G degrees revealed that the reaction of sulfosulfuron with DNA could proceed spontaneously, and the hydrogen bonding and van der Waals forces were essential to sulfosulfuron-ct-DNA binding, which was further verified by molecular docking study. Meanwhile, the electrostatic and hydrophobic interactions also played a supporting function for the interaction of sulfosulfuron with ct-DNA. The circular dichroism (CD) results exhibited a minor change in the secondary structure of ct-DNA during interaction process. Moreover, the conformation of sulfosulfuron had the obvious change after binding to DNA, which suggested that the flexibility of sulfosulfuron contributed to stabilizing the sulfosulfuron-ct-DNA complex.

• 单位
  浙江工业大学