Optimization of the ultrasonic-assisted extraction of trans-resveratrol and its glucoside from grapes followed by UPLC-MS/MS using the response surface methodology

作者：Guo, Jing; Zhao, Jinhui; Zhang, Mingjia; Sun, Zhiwei^*; Liu, Liyan^*

来源：Journal of Food Measurement and Characterization, 2022, 16(2): 1124-1136.

DOI：10.1007/s11694-021-01236-7

• Trans-resveratrol
• UPLC-MS
• MS
• Ultrasonic extraction
• Response surface methodology

Resveratrol with a high content in grapes has beneficial effects on inflammation, endothelial function, oxidative stress and cardiovascular and cerebrovascular diseases. Here, we propose a sensitive analytical method basing on the ultrasonic-assisted extraction followed by ultra-high performance liquid mass spectrometry to simultaneously determine trans-resveratrol (RES), trans-resveratrol 3-O-beta-D-glucuronide, trans-resveratrol 4MODIFIER LETTER PRIME-O-beta-D-glucuronide (R4G) in grapes. Single factor experiment and response surface methodology were used to optimize the pretreatment process of grape samples. The optimal conditions were as follows: extraction solvent-ethyl acetate, ultrasonic temperature 62 degrees C, ultrasonic time 20 min, ultrasonic power 90% and the volume of the extracted solvent 3500 mu L. The method showed the good linearity (r >= 0.9917), the satisfactory intra-day (%RSD <= 11.3%) and inter-day precision (%RSD <= 14.5%) and low limit of quantification (0.1 mu g/mL). This proposed method was successfully applied to determine RES, R3G, R4G in different grapes, achieving satisfactory recoveries (88.8-116.9%). Thus, this method established in this study is simple and time-saving, which will be helpful to the development and utilization of RES, R3G, R4G in grapes in food and pharmaceutical industries.

• 单位
  哈尔滨医科大学