Optical tweezers assisted analyzing and sorting of tumor cells tagged with fluorescence nanospheres in a microfluidic chip

作者：Zheng, Bei; Li, Cheng-Yu; Huang, Sha; Zhang, Zhi-Ling; Wu, Qiong-Shui^*; Pang, Dai-Wen^*; Tang, Hong-Wu^*

来源：Sensors and Actuators, B: Chemical , 2022, 368: 132173.

DOI：10.1016/j.snb.2022.132173

• Optical tweezers
• Fluorescence probe
• Microfluidic chip
• Cell screening
• Automatic detection

Due to the vital role of studying a single tumor cell, it is meaningful to establish a compatible means to realize the analysis and sorting tasks. Despite plentiful strategies have been developed, there is still a challenge to develop a fully integrated approach to be directly used in biological samples. As such, we present here a special fluorescence activated cell sorting platform assisted by optical tweezers (FACS-OT) in a microfluidic chip. To begin with, according to the significant differences in the protein expression level of cell surface, two kinds of cells are severally immunotagged with quantum dots embedded two-color fluorescent nanospheres. And then, by the home-built setup, different cells are stably and accurately detected one by one for single-cell level with two-color fluorescence resolved in the microfluidic chip. Finally, the home-built apparatus of FACS-OT is successfully used for highly precise deflection of targeted cells (deflection ratio of 95.7%) under the radiation of optical force. In addition, the results of simulation agree with our experiment, which offer a stable platform for further application. In line with above efforts, we establish a special platform by automatic analysis and deflection of single cell, offering a special strategy for studying heterogeneity in single cell.

• 单位
  武汉大学; 南开大学