Boron-Assisted Selective Citrulline Modification under Mild Conditions

作者：Zhao, Hailong; Shan, Aidong; Liang, Yunshi; Wu, Haiting; He, Yiting; Chen, Huihong; Zeng, Jiaxin; Gu, Jiangyong^*; Song, Jian-Ping^*; Qiu, Hong^*; Zhang, Jing^*

来源：Organic Letters, 2022, 24(34): 6351-6355.

DOI：10.1021/acs.orglett.2c02722

• DYNAMIC KINETIC RESOLUTION
• CHEMICAL BIOLOGY
• PROTEIN
• PHENYLGLYOXAL
• INHIBITION
• PROBE
• ACIDS

Protein citrullination is one type of protein post-translational modification. Previous methods entail the use of a strongly acidic condition (pH <1), which impedes its exploration under physiological and pathological conditions. Here, we developed a biocompatible method based on o-boron-assisted citrulline modification. We demonstrated that this method enables selective and mainly irreversible modification of citrulline residues under neutral conditions. We expect that it will provide a valuable tool for the study of protein citrullination.

• 单位
  广州中医药大学; 1; 中国科学院