Characterization of a New Bifunctional and Cold-Adapted Polysaccharide Lyase (PL) Family 7 Alginate Lyase from Flavobacterium sp.

作者：Zhou, Hai-Xiang; Xu, Shan-Shan; Yin, Xue-Jing; Wang, Feng-Long^*; Li, Yang^*

来源：Marine Drugs, 2020, 18(8): 388.

DOI：10.3390/md18080388

• alginate lyase
• bifunctional
• cold-adapted
• Flavobacteriumsp
• S02
• Yarrowia lipolytica

Alginate oligosaccharides produced by enzymatic degradation show versatile physiological functions and biological activities. In this study, a new alginate lyase encoding genealyS02fromFlavobacteriumsp. S02 was recombinantly expressed at a high level inYarrowia lipolytica, with the highest extracellular activity in the supernatant reaching 36.8 +/- 2.1 U/mL. AlyS02 was classified in the polysaccharide lyase (PL) family 7. The optimal reaction temperature and pH of this enzyme were 30 degrees C and 7.6, respectively, indicating that AlyS02 is a cold-adapted enzyme. Interestingly, AlyS02 contained more than 90% enzyme activity at 25 degrees C, higher than other cold-adapted enzymes. Moreover, AlyS02 is a bifunctional alginate lyase that degrades both polyG and polyM, producing di- and trisaccharides from alginate. These findings suggest that AlyS02 would be a potent tool for the industrial applications.

• 单位
  y; 青岛大学