Human mesenchymal stromal cells enhance the immunomodulatory function of CD8(+) CD28(-) regulatory T cells

作者：Liu Qiuli; Zheng Haiqing; Chen Xiaoyong; Peng Yanwen; Huang Weijun; Li Xiaobo; Li Gang; Xia Wenjie; Sun Qiquan^*; Xiang Andy Peng

来源：CELLULAR & MOLECULAR IMMUNOLOGY, 2015, 12(6): 708-718.

DOI：10.1038/cmi.2014.118

• antigens
• CD28
• CD8-positive T cells
• mesenchymal stromal cells
• regulatory T cells

One important aspect of mesenchymal stromal cells (MSCs)-mediated immunomodulation is the recruitment and induction of regulatory T (Treg) cells. However, we do not yet know whether MSCs have similar effects on the other subsets of Treg cells. Herein, we studied the effects of MSCs on CD8(+) CD28(-) Treg cells and found that the MSCs could not only increase the proportion of CD8(+) CD28(-) T cells, but also enhance CD8(+) CD28(-)T cells' ability of hampering naive CD4(+) T-cell proliferation and activation, decreasing the production of IFN-gamma by activated CD4(+) T cells and inducing the apoptosis of activated CD4(+) T cells. Mechanistically, the MSCs affected the functions of the CD8(+) CD28(-) T cells partially through moderate upregulating the expression of IL-10 and FasL. The MSCs had no distinct effect on the shift from CD8(+) CD28(+) T cells to CD8(+) CD28(-) T cells, but did increase the proportion of CD8(+) CD28(-) T cells by reducing their rate of apoptosis. Insummary, this study shows that MSCs can enhance the regulatory function of CD8(+) CD28(-) Treg cells, shedding new light on MSCs-mediated immune regulation.

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