CrWSKP1, an SKP1-like Gene, Is Involved in the Self-Incompatibility Reaction of "Wuzishatangju" (Citrus reticulata Blanco)

Authors：Li Peng; Miao Hongxia; Ma Yuewen; Wang Lu; Hu Guibing; Ye Zixing; Zhao Jietang; Qin Yonghua^*

Source：International Journal of Molecular Sciences, 2015, 16(9): 21695-21710.

DOI：10.3390/ijms160921695

• Citrus reticulata Blanco
• self-incompatibility
• SKP1
• genetic transformation

Plant S-phase kinase-associated protein 1 (SKP1) genes play crucial roles in plant development and differentiation. However, the role of SKP1 in citrus is unclear. Herein, we described a novel SKP1-like gene, designated as CrWSKP1, from Wuzishatangju (Citrus reticulata Blanco). The cDNA sequence of CrWSKP1 is 779 base pairs (bp) and contains an open reading frame (ORF) of 477 bp. The genomic sequence of the CrWSKP1 gene is 1296 bp with two exons and one intron. CrWSKP1 has high identity with SKP1-like genes from other plant species within two conserved regions. Approximately 85% of pollen tubes of self-pollinated CrWSKP1 transgenic tobaccos became twisted at four days after self-pollination. Pollen tube numbers of self-pollinated CrWSKP1 transformants entering into ovules were significantly fewer than that of the control. Seed number of self-pollinated CrWSKP1 transformants was significantly reduced. These results suggested that the CrWSKP1 is involved in the self-incompatibility (SI) reaction of Wuzishatangju.

• Institution
  中国热带农业科学院; 华南农业大学; 中国热带农业科学院椰子研究所; 中国农业大学