Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

作者：Zong, Weijian; Wu, Runlong^*; Chen, Shiyuan; Wu, Junjie; Wang, Hanbin; Zhao, Zhe; Chen, Guoqing; Tu, Rui; Wu, Danlei; Hu, Yanhui; Xu, Yangyang; Wang, Yao; Duan, Zhuoli; Wu, Haitao; Zhang, Yunfeng; Zhang, Jue; Wang, Aimin^*; Chen, Liangyi^*; Cheng, Heping

来源：Nature Methods, 2021, 18(1): 46-+.

DOI：10.1038/s41592-020-01024-z

We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420x420x180 mu m(3) at a lateral resolution of similar to 1 mu m. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.

• 单位
  北京大学; 南方医科大学; 中国医学科学院