In Vitro Characterization and Histological Evaluations of Paclitaxel-Loaded Nanofibrous Matrices, as Potential Local Therapeutic for Treating Breast Cancer: An In Vitro and In Vivo Study

摘要

In the current study, paclitaxel was loaded into electrospun gelatin nanofibers in order to produce a potential anti-cancer membrane. The developed system was studied for its cytotoxic effects against breast cancer MCF-7 cells. Further-more, biodegradation rate, microstructure, cell migration assay, and tensile strength were performed for the evaluation of anticancer local therapeutic. Study showed that, the electrospun membranes were composed of smooth and web-like nanofibers. In addition, the paclitaxel-loaded nanofibers were toxic against breast cancer cells and prevented their migra-tion. Real time PCR assay showed that the anticancer activity of the developed system might be through regulation of DJ-1/KLF 17 signalling pathway.

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