Engineering of salt-tolerant Shewanella aquimarina XMS-1 for enhanced pollutants transformation and electricity generation

作者：Zhang, Zong-Bin; Cheng, Zhou-Hua; Wu, Jing-Hang; Yue, Zheng-Bo; Wang, Jin^*; Liu, Dong-Feng^*

来源：Science of the Total Environment, 2022, 807: 151009.

DOI：10.1016/j.scitotenv.2021.151009

• Saline wastewater
• Shewanella aquimarina XMS-1
• Engineered strain
• Pollutant transformation
• Electricity generation
• 3D biohybrid system

Saline wastewater poses a challenge during bio-treatment process due to salinity affecting the physiological activity of microorganisms and inhibiting their growth and metabolism. Thus, screening and engineering the salt-tolerant strains with stronger performances are urgent. Shewanella aquimarina XMS-1, a salt-tolerant dissim-ilated metal reducing bacteria (DMRB), was isolated from seawater environment. Its ability for reducing pollut-ants and generating electricity was enhanced by overexpression of riboflavin synthesis pathway encoding genes from S. oneidensis MR-1 under salt stress. Furthermore, upon contact with graphene oxide (GO), the engineered strain XMS-1/pYYDT-rib with enhanced flavins synthesis could reduce GO and self-assemble to form a three-dimensional (3D) biohybrid system named XMS-1/flavins/rGO. This 3D biohybrid system significantly enhanced the EET efficiency of S. aquimarina XMS-1. Our findings provide a feasible strategy for treatment of salt-containing industrial wastewater contaminated by metal and organic pollutants.

• 单位
  中国科学院