MXene-Based Aptameric Fluorosensor for Sensitive and Rapid Detection of COVID-19

作者：Luo, Yao; Jiang, Xin; Zhang, Rong; Shen, Chen; Li, Mei; Zhao, Zhenzhen; Lv, Mengyuan; Sun, Shengjun; Sun, Xuping; Ying, Binwu^*

来源：Small, 2023, 19(23).

DOI：10.1002/smll.202301146

• cellular imaging
• COVID-19
• fluorosensors
• MXene nanosheets
• spike proteins

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-caused COVID-19 pandemic has rapidly escalated into the largest global health emergency, which pushes to develop detection kits for the detection of COVID-19 with high sensitivity, specificity, and fast analysis. Here, aptamer-functionalized MXene nanosheet is demonstrated as a novel bionanosensor that detects COVID-19. Upon binding to the spike receptor binding domain of SARS-CoV-2, the aptamer probe is released from MXene surface restoring the quenched fluorescence. The performances of the fluorosensor are evaluated using antigen protein, cultured virus, and swab specimens from COVID-19 patients. It is evidenced that this sensor can detect SARS-CoV-2 spike protein at final concentration of 38.9 fg mL(-1) and SARS-CoV-2 pseudovirus (limit of detection: 7.2 copies) within 30 min. Its application for clinical samples analysis is also demonstrated successfully. This work offers an effective sensing platform for sensitive and rapid detection of COVID-19 with high specificity.

• 单位
  电子科技大学; 四川大学