Content changes of Jiupei tripeptide Tyr-Gly-Asp during simulated distillation process of baijiu and the potential in vivo antioxidant ability investigation

摘要

Baijiu is the national liquor of China. Fermented grain (Jiupei) is the main material for baijiu distillation process. A tripeptide Tyr-Gly-Asp (YGD) with in vitro antioxidant ability was isolated from Jiupei. As one of the nonvolatile components, existence of Jiupei peptides after baijiu distillation is still unclear. Study aims to investigate the content changes of total peptides and YGD during the high temperature of distillation process, YGD stability in baijiu and its influence on baijiu characters. AAPH induced oxidative stress Sprague Dawley (SD) rat model was used to evaluate YGD antioxidant and anti-inflammatory activities in vivo. Results showed that YGD content changed under the high temperature of simulated distillation process, exist stably in baijiu and show little influence on baijiu quality. YGD was able to attenuate AAPH-induced oxidative stress by elevating the expressions of Nrf2/Keap1-p38MAPK/PI3K-MafK signaling pathway and downstream antioxidant enzymes. In addition, YGD significantly inhibited the overproduction of inflammatory cytokines and mediator caused by AAPH in rat serum. In light of these findings, YGD is a tripeptide with in vivo antioxidant activity isolated from Jiupei, which proves functional peptides in baijiu are traced from Jiupei and lay a foundation for the development of healthy baijiu.

关键词