A rare ?-8 fatty acid, cis-8,4,11-docosatrienoic acid rich microalgal strain isolated from a salt lake in Tibet Plateau br

作者：Han, Zhiping; Moh, Edward S. X.; Peng, Yuanhuai; Wang, Sheng; Jin, Bei; Huang, Ying; Ye, Jianzhi^*

来源：Algal Research-Biomass Biofuels and Bioproducts, 2022, 66: 102767.

DOI：10.1016/j.algal.2022.102767

• Plateau microalgae
• Desmodesmus
• Salt lake
• cis-8
• 4
• 11-docosatrienoic acid

A new green microalga was isolated from a salt lake in Tibet Plateau, China, classified as genus Desmodesmus under the family of Scenedesmaceae based on morphological and 18S rDNA sequencing. The species was able to grow at both 4 and 25 degrees C, due to differences to the biochemical composition. At 25 degrees C, protein content made up 50 % in dry biomass, while at 4 degrees C lipids made up 55 % of dry biomass. A poly unsaturated fatty acid, cis-8,11,14- docosatrienoic acid (C22:3, 8,11,14-DTA), was identified in D. sp. QL96 cultured under both temperature con-ditions. This fatty acid has been reported to be physiologically functional, however yield from natural sources was low and limited the related research and application. GC analysis indicated that 8,11,14-DTA was the third abundant fatty acid in D. sp. QL96, accounting for around 20 % out of the total lipid. The effects of environ-mental factors on the production of 8,11,14-DTA was studied and productivity of 8,11,14-DTA was optimized to achieve 10.91 mg/L per day. To our knowledge, the plateau alga is the most productive natural source of 8,11,14-DTA. This study provides the groundwork for further study and processing of this fatty acid.

• 单位
  中国科学院