Tapered Side-Polished Microfibre Sensor for High Sensitivity hCG Detection

作者：Luo, Wei; Liu, Bin^*; Liu, Juan; Wu, Tao; Liu, Qiang; Wang, Meng-Yu; Wan, Sheng-Peng; Yuan, Jinhui; Lu, Ping; Wang, Danling; He, Xing-Dao; Wu, Qiang

来源：IEEE SENSORS JOURNAL, 2022, 22(8): 7727-7733.

DOI：10.1109/JSEN.2022.3156284

• Optical fiber sensors
• Optical fibers
• Sensors
• Sensitivity
• Optical variables measurement
• Optical refraction
• Optical interferometry
• Optical microfiber
• biosensor
• side-polished optical fiber
• refractive index
• human chorionic gonadotropin (hCG)

A high sensitivity human chorionic gonadotropin (hCG) detection was conducted by a tapered sidepolished (TSP) optical fiber sensor. Experimentally, the TSP fiber sensor was made by side polishing a short section of single mode fiber to a D shape structure and tapering the D shape section to a small diameter (<10 mu m in the experiments). By functionalizing the primary antibody of hCG onto the TSP fiber surface, the sensor was used for detecting hCG concentration. Experimental results show that when the hCG concentration is 0.1 mIU/mL, the sensor has an average wavelength shift of 0.82 nm. The limit of detection (LoD) of the hCG is estimated 0.058 mIU/mL, assuming three times of maximum wavelength variation (3 x 0.15 = 0.45 nm) in Phosphate buffer saline (PBS) to the measurement limit. The specificity has also been tested by immersing the sensor into a mixed biomaterial solution (hCG - 1 mIU/mL, pig-IgG - 1 mu g/mL, Staphylococcus aureus -6 x 10(5) CFU/mL and Escherichia coli - 2.5 x 10(5) CFU/mL). The result showed that the TSP optical fiber sensor has excellent specificity. The biosensor has potential application in clinical/medical diagnostics, human health, environmental quality and food safety monitoring.

• 单位
  东北大学; 南昌航空大学; 华中科技大学; 1; 北京科技大学