科研之友
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摘要
This study investigated the relationship between the initial characteristics of poplar fibers changed by drying and its enzymatic digestibility (cellulose and xylan) in the whole hydrolysis process. The results showed drying has an inhibitory effect on enzymatic hydrolysis. Based on the results and statistical analysis, a drying index was proposed to represent the accessibility of the dried poplar fiber to enzymes, which is expressed as the ratio of water retention value to crystallinity index. Cellulose digestibility, xylan digestibility, and total substrate digestibility all showed a significantly linear positive correlation with the drying index in the whole hydrolysis process. The synergistic effect of xylanase in enzymatic cocktails changed regularly, but the weak synergistic effect always occurs first and then the strong synergistic effect. The difference in inhibition caused by different drying conditions increased first and then stabilized or decreased slowly, which was found in the regular changes of the slope in the linear relationship. Therefore, the substrate characteristics after swelling should have a certain relationship with its initial characteristics and change according to certain rules during enzymatic hydrolysis. This paper reveals the possible hydrolysis process, which will further promote the research into the mechanism of enzymatic hydrolysis.
