The amino-terminal region of dense granule protein 6 of Toxoplasma gondii stimulates IFN-γ production by microglia

作者：Qila Sa; Corinne Mercier; Marie-France Cesbron-Delauw; Yasuhiro Suzuki

来源：Microbes and Infection, 2020.

DOI：10.1016/j.micinf.2019.12.003

• Toxoplasma
• IFN-gamma
• Protective immunity
• Innate immunity
• Microglia
• Dense granule protein 6

We examined activities of dense granule proteins (GRAs), which Toxoplasma gondii secretes within infected cells, to stimulate microglial IFN-γ production in vitro. We identified that the N-terminal region (amino acids 41–152) of GRA6 (GRA6Nt) stimulates IFN-γ production by both a microglia cell line and primary microglia purified from the brains of uninfected adult mice. In contrast, neither of GRA1, GRA2, GRA5Nt, nor the carboxyl-terminal (amino acids 174–224) of GRA6 stimulated microglial IFN-γ production. GRA6Nt appears to be a target molecule of the sentinel function of microglia to detect cerebral proliferation of T. gondii and activate their IFN-γ production for facilitating the protective immunity to control the pathogen.

• 单位
  University of Kentucky College of Medicine