Efficient discovery of novel antimicrobials through integration of synthesis and testing in crude ribosome extract

作者：Sang, Zitai; Lu, Yongping; Zhou, Yuanzheng; Ju, Yuan; An, Qi; Shen, Silan; Shi, Jianyou; He, Jun; Yang, Tao^*; Luo, Youfu^*

来源：Chemical Communications, 2019, 55(42): 5886-5889.

DOI：10.1039/c9cc00144a

• CLICK CHEMISTRY

By coupling in situ [ 2+ 3] Huisgen cycloaddition with an in vitro transcription/ translation luminescence assay in a crude ribosomal extract, a robust and accurate high- throughput platform was successfully developed and applied for efficient identification of novel structural types of ribosomal inhibitors with antimicrobial activity against drug- resistant bacteria.

• 单位
  四川省医学科学院（四川省人民医院）; 电子科技大学; 四川大学; 成都中医药大学