EZH2 restricts Tcf7 DNA methylation and promotes TFH differentiation during acute viral infection

作者：Luo, Yuan; Li, Dan; Xie, Luoyingzi; Lei, Shun; Chen, Xiangyu; Wang, Cong; Yao, Dong; Li, Lin; Fang, Jingyi; Chen, Cheng; Yuan, Shijie; Li, Fei; Xie, Xiaorong; Zhang, Yan; Li, Zhirong; Hu, Li; Tang, Jianfang; Ye, Lilin; Wei, Zhengping^*; He, Ran^*

来源：Frontiers in Immunology, 2022, 13: 942465.

DOI：10.3389/fimmu.2022.942465

• LCMV
• T-FH cells
• EZH2
• DNA methylation
• Tcf7

Follicular helper T (T-FH) cells provide specialized help for B cells to ensure optimal humoral immunity. The histone methyltransferase EZH2, as a chromatin repressor, secures the T-FH differentiation by promoting T-FH lineage associated gene expression during acute viral infection, including Tcf7 and Bcl6. By using conditional deletion murine system, we observed that EZH2 ablation in CD4(+) T cells was accompanied by aberrant accumulation of DNA methyltransferases (DNMTs) DNMT1 and DNMT3B in T-FH cells. And the loss of EZH2 promoted aggravation of DNA methylation status at Tcf7 locus. Therefore, our findings suggested that EZH2 plays an important role in maintenance of hypomethylation at Tcf7 locus thus affecting T-FH differentiation during acute viral infection.

• 单位
  中国科学院研究生院; 华中科技大学; 南方医科大学