Anti-photoaging effect and mechanism of flexible liposomes co-loaded with apigenin and doxycycline

作者：Liu, Chang^*; Guo, Xiao; Chen, Yutong; Zhao, Meijun; Shi, Shuai; Luo, Zheng; Song, Jian; Zhang, Zhihong; Yang, Wenchuang; Liu, Keyi

来源：Biomedicine and Pharmacotherapy, 2023, 164: 114998.

DOI：10.1016/j.biopha.2023.114998

• Apigenin
• Doxycycline
• Flexible liposomes
• Photoaging
• Matrix metalloproteinases

Prolonged exposure to UV light can lead to photo-ageing of the skin. Therefore, the development and application of anti-photoaging drugs is urgent. In this study, we co-loaded apigenin (Apn) and doxycycline (Doc), a broadspectrum inhibitor of matrix metalloproteinases (MMPs), into flexible liposomes to exert anti-photoaging effects by combating oxidative stress, anti-inflammation, reducing the activation of MMPs and preventing collagen loss. The results showed that we prepared a flexible liposome (A/D-FLip) containing Apn and Doc. Its appearance, particle size and Zeta potential were normal and it had good encapsulation efficiency, drug loading, in vitro release and transdermal efficiency. In cellular experiments, A/D-FLip could inhibit oxidative stress damage, reduce inflammatory factors and decrease the activation of MMPs in Human immortalized keratinocytes (HaCaT) cells; in animal experiments, A/D-FLip could inhibit skin damage and reduce skin collagen loss by decreasing the activation of MMPs, thus inhibiting skin photoaging in mice. In conclusion, A/D-FLip has good anti-photoaging effects and it has the potential to become an effective skin care product or drug against UV damage and skin photoaging in the future.

• 单位
  哈尔滨医科大学