Narrative review: blood and tumor biomarker testing in non-small cell lung cancer without an oncogenic driver

作者：Zhang, Xingxing; Jiao, Pengbo; Zhang, Ming; Wu, Peng; Zhang, Yufeng; Wang, Yiwei; Xu, Kaiyan; Yu, Jiazhou; Ma, Liping^*

来源：Bioresource Technology, 2023, 370: 128578.

DOI：10.1016/j.biortech.2023.128578

• Anaerobic co -digestion
• Organic loading rate
• Hydraulic retention time
• Influencing mechanisms
• Metagenomic binning

This study provided novel insights into the effects of organic loading rate (OLR) and hydraulic retention time (HRT) on thermophilic anaerobic co-digestion of food waste and sewage sludge. The obtained maximum methane (CH4) yield of 328 +/- 4 mL CH4/g CODfed at HRT of 15 days (OLR = 5.8 g VS/L/d) was partly attributable to the enhanced acidogenesis, acetogenesis, and methanogenesis phases. The increased key enzyme activities, particularly acetate kinase (improved by 5.2-fold), providing substantial methanogenic substrates for efficient CH4 production. The functional syntrophs that were related to syntrophic decarboxylation, novel acetate oxidation & reductive acetyl-CoA, and beta-oxidation pathways could drive trophic interactions with methanogens. This markedly stimulated hydrogenotrophic Methanoculleus thermophilus metabolism and concomitantly enriched mixotrophic Methanosarcina thermophila. The distinctive cross-feeding interspecies interactions significantly affected the assembly and dynamics of thermophilic consortia. These findings shed light on the physicochemical and microbial mechanisms of HRT- and OLR-dependent enhancement of methanogenesis.