Brachybacterium huguangmaarense sp nov., isolated from Lake sediment

摘要

A Gram-stain positive strain, M1(T), was isolated from the sediment of Maar Lake in Zhanjiang, Guangdong Province, China. The diagnostic cell-wall diamino acid was meso-diaminopimelic acid, and mycolic acids were not detected. The polar lipid profile of strain M1(T) consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unknown glycolipid. The predominant quinone was MK-7, with MK-6 as a minor component. The major fatty acids were anteiso-C-15:0 and anteiso-C-17:0, with iso-C-18:0 as a minor component. The DNA G+C content of the genomic DNA was 71.0 mol%. 16S rRNA gene sequence analysis showed that strain M1(T) belongs to the family Dermabacteraceae, sharing highest sequence similarity with Brachybacterium nesterenkovii JCM 11648(T) (98.1%). Furthermore, a combination of DNA DNA relatedness and physiological and biochemical properties indicated that the novel strain could be readily distinguished from its closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain M1(T) represents a novel species of the genus Brachybacterium, for which the name Brachybacterium huguangmaarense sp. nov. is proposed. The type strain is M1(T) (=CCTCC AB 2012866(T)=DSM 26370(T)).

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