In vitro assessment of the DNA damage and senility of CD1 17+stem cells collected from diabetic mice

作者：She, Q-Y; Zhu, Y.; Chen, G.; Liu, Y.; Ruan, C-X; Wang, Q.; Sheng, X.; Deng, B.; Liang, H-X; Liu, Y-T; Young, C. A.; Qin, S-L^*

来源：European Review for Medical and Pharmacological Sciences, 2022, 26(4): 1084-1090.

• Diabetes
• CD1 17+BMCs
• DNA damage
• Senility

OBJECTIVE: Angiogenesis impairment is a common feature of diabetes mellitus (DM), whereas CD117+ bone marrow cells (BMCs) injury might be responsible for such complication. In this study, we studied the effect of hyperglycemia on the DNA damage and senility of CD117+ bone marrow cells. @@@ MATERIALS AND METHODS: We isolated CD117+ BMCs from the Streptozotocin (STZ) induced diabetes and healthy control mice. Oxidative stress was detected by flow cytometric analysis. gamma-H2AX, which is the DNA damage mark, was detected by using Western blotting and immunofluorescence histochemistry. We also detected the expression of gamma-H2AX and p16 by using Western blotting. @@@ RESULTS: Compared with the control mice. the level of reactive oxygen species (ROS) was increased significantly in the CD117+ BMCs collected from the diabetic mice (p<0.05), and the percentage of gamma-H2AX positive cells was higher significantly (p<0.01). The expression of gamma-H2AX and p16 was increased significantly in the CD117+ BMCs from the diabetic mice. @@@ CONCLUSIONS: Our experiments demonstrated the oxidative stress in CD117+ BMCs under DM conditions, while accelerating the DNA damage and senility in CD117+ BMCs as well.

• 单位
  中山大学; 南方医科大学; 5