3-Pentanol glycosides from root nodules of the actinorhizal plant Alnus cremastogyne

作者：Xu, Yingting; Xu, Yingchao; Huang, Zhengwan; Luo, Yucai; Gao, Ruanling; Xue, Jinghua; Lin, Changli; Pawlowski, Katharina; Zhou, Zhongyu^*; Wei, Xiaoyi

来源：Phytochemistry, 2023, 207: 113582.

DOI：10.1016/j.phytochem.2022.113582

• Alnus cremastogyne
• Betulaceae
• Root nodules
• 3-Pentanol glycosides
• 3-Pentanol

Alnus cremastogyne Burkill (Betulaceae), an actinorhizal plant, can enter a mutualistic symbiosis with Frankia species that leads to the formation of nitrogen fixing root nodules. Some primary metabolites (carbohydrates, dicarboxylic acids, amino acids, citrulline and amides) involved in carbon and nitrogen metabolism in actino-rhizal nodules have been identified, while specialized metabolites in A. cremastogyne root nodules are yet to be characterized. In this study, we isolated and identified three undescribed 3-pentanol glycosides, i.e., 3-pentyl alpha-L- arabinofuranosyl-(1 ''-*6 ')-beta-D-glucopyranoside, 3-pentyl alpha-L-rhamnopyranosyl-(1 ''-*6 ')-beta-D-glucopyranoside, and 3-pentyl 6 '-(3-hydroxy3-methylglutaryl)-beta-D-glucopyranoside, as well as seventeen known compounds from A. cremastogyne root nodules. 3-Pentanol glycosides are abundantly distributed in root nodules, while they are distributed in stems, roots, leaves and fruits at low/zero levels. A. cremastogyne plants treated by root nodule suspension emit 3-pentanol. This study enriches the knowledge about specialized metabolites in the actinorhizal host, and provides preliminarily information on the signal exchange in the actinorhizal symbiosis between A. cremastogyne and Frankia.

• 单位
  中国科学院研究生院; 广东省农业科学院