A novel strategy for rapid real-time chiral discrimination of enantiomers using serum albumin functionalized QCM biosensor

作者：Su Wen Cui; Zhang Wei Guang^*; Zhang Sheng; Fan Jun; Yin Xia; Luo Miao Li; Ng Siu Choon

来源：Biosensors and Bioelectronics, 2009, 25(2): 488-492.

DOI：10.1016/j.bios.2009.06.040

• QCM
• Serum albumin
• Biosensor
• Chiral discrimination
• UV/FL Spectra

A novel and effective method has been developed for chiral discrimination using a quartz crystal microbalance (QCM) biosensor with self-assembled bovine serum albumin (BSA) or human serum albumin (HSA). The successfully constructed QCM chiral biosensors exhibited rapid and real-time enantioselective recognition. The QCM chiral discrimination factor (alpha(QCM)) can be calculated through resonance frequency shifts in response to five pairs of enantiomers. Moreover, the interactions between these ten enantiomers and two serum albumins (SA) were investigated in detail by means of ultraviolet-visible (UV-vis) and fluorescence (FL) spectra. The results indicated that the discrimination ability were quite different between BSA and HSA. R,S-1-(3-Methoxyphenyl)ethylamine (R,S-3-MPEA) and R,S-1-(4-methoxyphenyl)ethylamine (R,S-4-MPEA) can be easily differentiated by the BSA sensor, while the selectivity of the HSA sensor for R,S-tetrahydronaphthylamine (R,S-TNA), R,S-2-octanol (R,S-2-OT) and R,S-methyl lactate (R,S-MEL) was higher than that of the BSA sensor. The UV and FL spectra indicated the formation of a complex between SA and enantiomers and strong fluorescence quenching through static quenching mechanism. The indepth study demonstrated that the calculated UV/FL discrimination factors (alpha(UV) and alpha(FL)) were consistent with the QCM experimental results (alpha(QCM)).

• 单位
  华南师范大学; 南阳理工学院