Osa-miR162a Enhances the Resistance to the Brown Planthopper via α-Linolenic Acid Metabolism in Rice (Oryza sativa)

作者：Chen, Jie^*; Liu, Qin; Yuan, Longyu; Shen, Wenzhong; Shi, Qingxing; Qi, Guojun; Chen, Ting; Zhang, Zhenfei^*

来源：Journal of Agricultural and Food Chemistry, 2023, 71(31): 11847-11859.

DOI：10.1021/acs.jafc.3c02637

• rice
• brown plant hopper
• microRNA
• miR162a
• resistance

The brown planthopper (BPH) is the most serious pestcausing yieldlosses in rice. MicroRNAs (miRNAs) are emerging as key modulatorsof plant-pest interactions. In the study, we found that osa-miR162ais induced in response to BPH attack in the seedling stage and tunesrice resistance to the BPH via the & alpha;-linolenic acid metabolismpathway as indicated by gas chromatography/liquid chromatography-massspectrometry analysis. Overexpression of osa-miR162a inhibited thedevelopment and growth of the BPH and simultaneously reduced the releaseof 3-hexenal and 3-hexen-1-ol to block host recognition in the BPH.Moreover, knockdown of OsDCL1, which is targetedby osa-miR162a, inhibited & alpha;-linolenic acid metabolism to enhancethe resistance to the BPH, which was similar to that in miR162a-overexpressingplants. Our study revealed a novel defense mechanism mediated by plantmiRNAs developed during the long-term evolution of plant-hostinteraction, provided new ideas for the identification of rice resistanceresources, and promoted a better understanding of pest control.

• 单位
  中山大学; 广东省农业科学院