Improvement of the stability and anti-AGEs ability of betanin through its encapsulation by chitosan-TPP coated quaternary ammonium-functionalized mesoporous silica nanoparticles

作者：Xu, Jiahao; Lai, Huining; You, Lijun; Zhao, Zhengang^*

来源：International Journal of Biological Macromolecules, 2022, 222: 1388-1399.

DOI：10.1016/j.ijbiomac.2022.09.239

• Betanin
• Chitosan
• Mesoporous silica nanoparticles

Mesoporous silica nanoparticles (MSNPs) are widely used as encapsulation materials, however, low encapsula-tion ability and the leakage and inactivation of the encapsulated components are two major disadvantages that limit their applications. Quaternary ammonium-functionalization and chitosan-sodium tripolyphosphate (CS-TPP) coating are used in this study to overcome these disadvantages. Betanin is a bioactive ingredient, but it is easily degraded. In this work, a new type of CS-TPP coated quaternary ammonium-functionalized MSNPs (CS@QAMSNPs) were synthesized by a reversed-phase microemulsion method, and betanin was encapsulated therein. The results of SEM, TEM, and FTIR of CS@QAMSNPs showed that MNSPs were functionalized with quaternary ammonium and coated with CS-TPP. The loading capacity of betanin-CS@QAMSNPs was 21.66 %, while that of betanin-MSNPs was 2.95 %. After encapsulation by CS@QAMSNPs, over 65 % of the antioxidant activity of betanin was retained after high-temperature and alkaline treatment, and 84.24 % of betanin was retained after ultraviolet-radiation treatment, implying an improvement in the stability of betanin. Cell viability was over 80 % in the presence of betanin and encapsulating materials, indicating their good food safety. The highest inhibition rate of betanin-CS@QAMSNPs in advanced glycation end-products in the BSA-fructose and BSA-MGO model was 47.39 % and 15 %, which was higher than those of betanin (BSA-fructose:11.38 %, BSA-MGO:0.83 %).