Colourful fluorescence-based carbon dots for tumour imaging-guided nanosurgery

作者：Shang, Wenting; Xia, Xueer; Lu, Ningning; Gao, Pengli; Peng, Li; Liu, Yu; Deng, Han; Jiang, Jingying^*; Li, Zhou^*; Liu, Jianhua^*

来源：Nanomedicine-Nanotechnology Biology and Medicine, 2022, 45: 102583.

DOI：10.1016/j.nano.2022.102583

• Carbon dots
• Fluorescent probe
• Multicolour
• cancer therapy
• Nano-theranostic

Fluorescent-intraoperative navigation is a visual technique that allows surgeons to accurately distinguish malignant and normal tissues during surgery. It has the advantages of immediacy, high resolution, and high specificity. However, a single fluorescent source cannot provide sufficient surgical information. Multicolour carbon dots (CDs) are more suitable since they provide outstanding water solubility, photo -stability, and multicolour-fluorescence imaging. Here, we prepared an optical probe with CD-based multicolour-fluorescence imaging via a hydrothermal method. CDs can be endocytosed by tumour cells, and after intravenous injection, they can effectively accumulate at the tumour site. In a pancreatic cancer mouse model, we demonstrated the multicolour-fluorescence imaging capabilities of CDs, which aided the accurate resection of tumours under fluorescent-intraoperative navigation. Stereoscopic fluorescence microscopy imaging and H&E staining proved that the removed tissue belonged to the pancreatic tumour. This study emphasizes the potential of CDs for fluorescence-guided intraoperative resection and expands the application of CDs in biological fields.

• 单位
  南方医科大学; 中国科学院; 北京航空航天大学; 广东省人民医院