微量生物物证提取套装在现场微量血痕DNA检验中的应用

作者：Ba Hua-Jie; Jin Ming; Shi Jin-Wei; Zhu Ai-Hua; Ma Jun

来源：Journal of Forensic Medicine, 2021, 37(1): 65-68.

DOI：10.12116/j.issn.1004-5619.2020.500303

• Bloodstain samples
• Forensic genetics
• Scene
• Trace Evidence Collection kit

Objective: To evaluate the effects of DNA examination of trace bloodstain samples from the scene collected with Trace Biological Evidence Collection kit. Methods: Venous blood was made into bloodstains on the ground. The trace bloodstain samples were collected with Trace Biological Evidence Collection kit and common methods, respectively. DNA examination of trace bloodstain samples(50 from each group) was conducted on the constant temperature shaker for 2, 24, 48, 72, and 96 h, respectively, and the examination results of every group were compared. Results: When the trace bloodstain samples were placed on the constant temperature shaker for 24, 48, 72, and 96 h, the DNA detection rates in the group which used Trace Biological Evidence Collection kit (100.00%, 100.00%, 100.00%, 96.00%) were significantly higher than those in the group using common methods (62.00%, 26.00%, 10.00%, 0), the differences had statistical significance(P<0.05). When the trace bloodstain samples were placed on the constant temperature shaker for 2 h, the differences of DNA detection rates between the two groups had no statistical significance (P>0.05). Conclusion: The Trace Biological Evidence Collection kit can effectively improve DNA detection rate and extend detection time limit for trace bloodstain samples from the scene that have been stored for a relatively long time.