-808 nm-activated Ca2+ doped up-conversion nanoparticles that release no inducing liver cancer cell (HepG2) apoptosis

作者：Fa, Xinmeng; Lin, Shaowei; Yang, Jianghua; Shen, Chong; Liu, Yuanli; Gong, Yongyang; Qin, Aimiao; Ou, Jun^*; Resch-Genger, Ute^*

来源：Methods and Applications in Fluorescence, 2022, 10(2): 024003.

DOI：10.1088/2050-6120/ac5524

• Ca2+ doped and Nd3+ sensitized UCNPs
• NIR-UV up-conversion fluorescence
• porous SiO2
• light-triggered NO release
• cancer cell apoptosis

A near-infrared (NIR) light-triggered release method for nitric oxide (NO) was developed utilizing core/shell NaYF4: Tm/Yb/Ca@NaGdF4: Nd/Yb up-conversion nanoparticles (UCNPs) bearing a mesoporous silica (mSiO(2)) shell loaded with the NO donor S-nitroso-N-acetyl-DL-penicillamine (SNAP). To avoid overheating in biological samples, Nd3+ was chosen as a sensitizer, Yb3+ ions as the bridging sensitizer, and Tm3+ ions as UV-emissive activator while co-doping with Ca2+ was done to enhance the luminescence of the activator Tm3+. NO release from SNAP was triggered by an NIR-UV up-conversion process, initiated by 808 nm light absorbed by the Nd3+ ions. NO release was confirmed by the Griess method. Under 808 nm irradiation, the viability of the liver cancer cell line HepG2 significantly decreased with increasing UCNPs@mSiO(2)-SNAP concentration. For a UCNPs@mSiO(2)-SNAP concentration of 200 mu g ml(-1), the cell survival probability was 47%. These results demonstrate that UCNPs@mSiO(2)-SNAP can induce the release of apoptosis-inducing NO by NIR irradiation.

• 单位
  桂林理工大学