Nanoparticle Spikes Enhance Cellular Uptake via Regulating Myosin IIA Recruitment

摘要

Spike-like nanostructures are omnipresent in naturaland artificialsystems. Although biorecognition of nanostructures to cellular receptorshas been indicated as the primary factor for virus infection pathways,how the spiky morphology of DNA-modified nanoparticles affects theircellular uptake and intracellular fate remains to be explored. Here,we design dually emissive gold nanoparticles with varied spikiness(from 0 to 2) to probe the interactions of spiky nanoparticles withcells. We discovered that nanospikes at the nanoparticle regulatedmyosin IIA recruitment at the cell membrane during cellular uptake,thereby enhancing cellular uptake efficiency, as revealed by dual-modality(plasmonic and fluorescence) imaging. Furthermore, the spiky nanoparticlesalso exhibited facilitated endocytosis dynamics, as revealed by real-timedark-field microscopy (DFM) imaging and colorimetry-based classificationalgorithms. These findings highlight the crucial role of the spikymorphology in regulating the intracellular fate of nanoparticles,which may shed light on engineering theranostic nanocarriers.

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