Electrochemical Enzyme Sensor Based on the Two-Dimensional Metal-Organic Layers Supported Horseradish Peroxidase

作者：Xiong, Yu; Wang, Chao; Wu, YuanFei; Luo, Chunhua^*; Zhan, Dongping; Wang, Shizhen^*

来源：MOLECULES, 2022, 27(23): 8599.

DOI：10.3390/molecules27238599

• electrochemical enzyme sensor
• metal-organic layers
• horseradish peroxidase
• hydrogen peroxide

Metal-organic frames (MOFs) have recently been used to support redox enzymes for highly sensitive and selective chemical sensors for small biomolecules such as oxygen (O-2), hydrogen peroxide (H2O2), etc. However, most MOFs are insulative and their three-dimensional (3D) porous structures hinder the electron transfer pathway between the current collector and the redox enzyme molecules. In order to facilitate electron transfer, here we adopt two-dimensional (2D) metal-organic layers (MOLs) to support the HRP molecules in the detection of H2O2. The correlation between the current response and the H2O2 concentration presents a linear range from 7.5 mu M to 1500 mu M with a detection limit of 0.87 mu M (S/N = 3). The sensitivity, reproducibility, and stability of the enzyme sensor are promoted due to the facilitated electron transfer.

• 单位
  厦门大学; 1